Quantitative analysis of free amino acids in Phaseolus vulgaris L. seeds
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This study analyzed the free amino acid composition of six Phaseolus vulgaris L. cultivars using high-performance liquid chromatography. The results revealed considerable variation among cultivars, with physiologically important amino acids such as aspartic and glutamic acids being predominant across all samples. Notably, the cultivar Belaya fasol exhibited an exceptionally high glutamine content (8.47 mg/g), while alanine, a key component of energy metabolism, showed consistently elevated levels (3.95–7.96 mg/g) in all cultivars. Essential amino acids, including leucine, isoleucine, threonine, and valine, displayed cultivar-specific patterns, reflecting differences in protein quality and nutritional potential. The findings highlight the significant biochemical diversity within common bean germplasm and underscore the nutritional value of these cultivars. Furthermore, the identified amino acid profiles provide a valuable basis for breeding programs aimed at biofortification, improved protein quality and the development of high-value common bean varieties for both food security and health-oriented applications.
